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Abstract
Vaccination is the cost-effective preventive measure for influenza; however, at times with antigenic mismatch, annual revision of seasonal influenza vaccine components due to constant-evolving nature of the virus can potentially occur with the vaccine target HA. This research project endeavors to pioneer advancements in the realm of influenza vaccine design by adopting a consensus-based methodology centered on the HA protein sequence of the H3N2 influenza strain. The approach involves the creation of synthetic HA proteins that represent the diversity of the H3N2 HA population, aiming to enhance cross-reactivity and broaden vaccine coverage. To achieve this, consensus sequences were meticulously generated for the entire HA sequence, as well as the HA head and HA stem regions which were strategically designed according to diverse phylogenetic trees, antigenic clusters, and vaccine strains. A total of 97 distinct sequences were derived, out of which 30 designed sequences were finalized to test in-vitro.