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Abstract
To uncover information regarding the condition of a disease or ailment caused by dysregulation in biochemical pathways, it is imperative to utilize biomarkers to assess and quantitate changes in metabolite concentrations in response to atypical conditions. Analytical methods provide the means to quantitate analytes and uncover information for various studies including metabolic pathways, pharmacokinetics of a particular metabolite and its onset and duration of action. In Chapter 1, an introduction to liquid chromatography and mass spectrometry is presented. Chapter 2 is a literature review of the chromatographic methods used for the quantitation of acyl-CoAs with an emphasis on liquid chromatography tandem mass spectrometry methods.