Electrolyzed oxidative water applied using electrostatic spraying is an effective means of eliminating pathogenic and indicator populations of bacteria from hatching eggs. Using this method in a pilot scale hatchery, the percentage of chickens that were colonized with Salmonella was dramatically reduced. These studies demonstrate that the use of EO water in combination with electrostatic spraying may provide a practical and inexpensive way for the industry to significantly lower the number of birds that arrive at the processing plant contaminated with Salmonella.