The phenomenon of DNA condensation has been studied for decades, often as a model of DNA packaging within viruses and chromosomes, due to its medical importance as a key step in gene therapy. The approach of using oligopeptides and low molecular weight natural polyamines as DNA packaging systems has received a lot of interest due to the biochemical knowledge that in vivo, DNA is usually associated with peptides composed of 5-20 amino acids in viruses and polyamines such as spermine and spermidine in chromosomes that facilitate DNA condensation and packaging. Amphiphilic polymers such as Poly(L-lysine)-b-Poly(ethylene glycol) and poly(ethylenimine)-alt-Poly(ethylene glycol) have also shown a lot of potential as DNA condensation and packaging systems. In this study, utilization of natural polyamines, oligopeptides, and ampiphilic polymers in DNA condensation, packaging and their potential application in gene therapy is explored.