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Abstract
The present study determined the effects of the lethal acrodermatitis (LAD) mutation, dietary zinc deficiency, and exercise on the protein composition of lipid rafts. The lipid rafts from LAD dog kidney and rat liver were separated using the ice-cold Triton X-100. Two dimensional gel electrophoresis (2DE) was utilized for identifying differential protein expression in these membrane fractions. Rats were fed diets containing 5 and 30 ppm zinc, and were separated into exercise and sedentary groups for 42 days. Differences were assessed using the general linear model (GLM) and the students unpaired t test with repeated measures. One protein was up regulated with 5 ppm zinc. One protein was down regulated during exercise. One protein spot was decreased in the affected LAD kidney lipid rafts. In summary, these results will further our understanding of the LAD mutation and the role of exercise in altering zinc homeostasis.