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Abstract
Utilization of sex-sorted semen in dairy heifers has become a frequent topic of research due to its positive attributes to a herd, but the full adoption of this technology into practice has been overshadowed by low economic gain due to lower conception rates compared to conventional semen. In this study, deep intra-cornual insemination ipsilateral to the ovary with the preovulatory follicle was used to evaluate the effect of site of semen deposition with sexed semen on pregnancy per timed artificial insemination (P/TAI). The results show that deep intra-cornual insemination resulted in greater P/TAI compared to deposition in the uterine body (56.7%; 44.32%: P = 0.038). Different inseminators had an effect on P/TAI (P = 0.09), and there was a higher numeric tendency for pregnancy in the left versus the right horn (66.3%; 53.9%). This suggests that deep intra-cornual insemination may help overcome the lower P/TAI previously achieved with sex-sorted semen.