Renewed interest in the study of auxin herbicides (WSSA group 4) is increasing due to the release of genetically engineered crop varieties that are tolerant to PRE and POST applications of dicamba. The development of auxin resistant crops was in response to the discovery of herbicide resistant weed species to glyphosate and other herbicides that resulted in yield loss. Efficacy for all herbicide treatments was affected by the times of application. Daylight applications resulted in greater overall control, with the noon to 1-hr prior to sunset application timings exhibiting the greatest control of all weed species observed. Plant stress indicators were also observed at higher rates for a noon-time application than for fully dark-adapted applications using dicamba and glyphosate. These results could support expanding the timing of application to increase efficacy while reducing off target herbicide effects and help growers make informed herbicide application decisions.