Research was conducted on golf courses in Johns Creek, Milton, and Griffin GA from May 2018 to October 2019 to evaluate long-term effects of wetting agents, plant growth regulators, and biological products on microbial abundance and function using quantitative polymerase chain reaction, phosphatase, urease, and soil respiration assays. In the putting green trial Revolution improved phosphatase activity and whereas Cutless enhanced phosphatase activity in the fairway. In the fairway, Cutless and Primo Maxx depressed urease activity over time as compared with the Control and Trimmit. The biologicals overall did not show much significant differences between the two trials as compared to the control with the exception of BP2 applied subsurface resulting in enhanced soil respiration, urease activity, and ammonia oxidizers.