Ochratoxin A (OTA) is the toxic secondary metabolite produced by several fungal species, including Aspergillus ochraceus and Penicillium verrucosum. OTA has been reported to cause nephrotoxicity, and various reports have suggested that OTA might be involved in the occurrence of Balkan endemic nephropathy (BEN). In this thesis research, we evaluated the toxicological effects of OTA to C. elegans based on multiple toxic endpoints, including growth, reproduction, and locomotion, using high-throughput assays. Our results showed that growth and reproduction in C. elegans were significantly reduced upon the exposure to OTA with concentration-dependent manners. In addition, the result of the locomotion experiment in C. elegans exposure of OTA displayed a time-dependent decrease in the motility of worms, but, as time went on, a concentration-dependent increase in the motility of worms was indicated as compared to the untreated control. So far, no study has reported the toxic effects of OTA in C. elegans. These results suggested that C. elegans can serve as a good model organism for evaluation of toxic effects on OTA and mechanistic studies of OTA-induced adverse health effects.