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Abstract
Light Sheet Microscopy is a recently revitalized field of fluorescence microscopy that offers high speed imaging over a wide field of view (FOV). Light sheet microscopes are often used for biological studies of model organisms such as zebrafish because of they offer high-speed imaging of in vivo events, high resolution, and optical sectioning. The organisms imaged with a light sheet microscope often serve as models that can for human biology. However, light sheet microscopy is limited to 2D image acquisition. By moving the sample through the focal plane, 3D data acquisition is possible; however this mechanical movement is often too slow to accurately capture in vivo events. By pairing the light sheet microscope with an electric tunable lens (ETL), high speed volumetric imaging can be achieved for the purpose of studying in vivo events. We demonstrate the system by imaging the Central Nervous System (CNS) of Zebrafish embryos while neuronal events occurred. We demonstrate imaging the brain of the zebrafish at a volume of 400 by 400 by 100µm3 at a rate of 0.6Hz. at a rate of 0.6Hz.