Isoprenol is an excellent alternative for fuel sources and a precursor for many commercial chemicals in industrial applications. Microbial production of isoprenol via the mevalonate pathway has been previously optimized in E. coli, resulting in approximately 2.23 g/L titers using the promiscuous activity of known phosphatases, NudB and AphA, and “IPP-Bypass system. However, limitations achieving optimal production still exist. To overcome these limitations, we have developed a more efficient synthetic pathway for enhanced isoprenol production by expanding upon existing metabolic pathways in E. coli by incorporating the upper MVA pathway into the genome and regulating lower pathway components using the “IPP-Bypass” and plasmid-based expression systems. In this work we have uncovered a new phosphatase, yigL, capable of isoprenol production with increased titers of 2.92 g/L. This novel finding combined with future work and applications presented herein have the potential to achieve the new industrial standard in isoprenol production.