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Abstract
Over the years, there has been a gap in literature regarding the accuracy of themethods used to calculate metabolizable energy (ME) values for feedstuffs. Previous studies
report ME values for feedstuffs that are not in agreement with other reported values. The
differences in ME values of feedstuffs can be influenced by either bird-related, diet-related,
and methodology-associated factors. However, the current methodologies do not consider the
influence that these factors have on determining the ME values of feedstuffs. Understanding
the factors that can influence the ME of feedstuffs can help reduce the variation in feedstuffs
ME values. This study aimed to 1) Evaluate the influence of reference diet on ME of soybean
meal and canola meal using difference vs. regression assay methods, and 2) Evaluate the
influence of adaptation length on ME of corn and barley when supplemented with or without
carbohydrase using total collection vs. index assay methods.