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Abstract
Monkeyflowers (genus Mimulus) serve as model species used in studies on adaptation, speciation, and evolution. The implementation of functional genomics is necessary to increase the utility of Mimulus as a model system. In this context, the development of reliable tissue culture and Agrobacterium-mediated transformation strategies are essential. This study is to determine suitable genotypes for tissue culture and ultimately provide a protocol for Agrobacterium-mediated transformation in the yellow flower clade of Mimulus. 15 inbred lines from the yellow-flowered clade of Mimulus were tested for their performance in tissue culture using two regeneration systems. From this evaluation, suitable genotypes were chosen for a subsequent experiment testing parameters of transformation including various Agrobacterium strains and multiple days of cocultivation.