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Abstract

My dissertation is comprised of research from two distinct fields: fungal cellularbiology and discipline-based education research (DBER). The fungal cellular biology portion of my dissertation focuses on mechanisms of nuclear migration during rice cell invasion and proliferation by the blast fungus, Magnaporthe oryzae. Specifically, I characterized the involvement of the mitotic spindle in mediating nuclear migration at three different stages of rice blast infection: nuclear migration through the germ tube of developing appressoria, nuclear migration through the narrow penetration peg, and nuclear migration through the narrow invasive hyphal peg. The structure of the nuclear envelope is also described during nuclear migration through the germ tube of developing appressoria. Conserved kinesin motor proteins, MoKin5 and MoKin14, were identified, and their function in mediating nuclear migration through the penetration peg was analyzed using an overexpression approach. These studies provide fundamental knowledge about the cellular biology of the rice blast fungus during the early stages of rice cell invasion and colonization that can serve as a basis for future research. My DBER studies focus on the self-advocacy experiences of students with ADHD and specific learning disabilities (SLD) in undergraduate STEM courses. Research interviews with 25 STEM majors with ADHD and SLD were conducted. From an in-depth qualitative analysis, a revised conceptual model of self-advocacy emerged. This revised self-advocacy model is tailored to STEM undergraduates with ADHD and SLD. We utilized this revised self-advocacy model to conduct an additional analysis of the interview data. In this study, the factors that influence self-advocacy were identified. We proposed a model to understand how these factors interact to support or hinder selfadvocacy within undergraduate STEM courses. Our revised model of selfadvocacy provides implications for both future research and teaching.

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