Suppressor of Female Function, SOFF, is a sex determining gene in garden asparagus, Asparagus officinalis L., though its molecular function is unknown. SOFF is a member of the Domain of Unknown Function family 247. Alignment of SOFF with a closely related paralog in A. officinalis indicates the possibility of two splice variants: one with a membrane-spanning domain and another with an extra exon relative to other DUF247 genes. Through RNA-seq analysis of male and female flower buds collected at different developmental stages I could not unambiguously validate these two splice variants. Development of a transformation protocol would enable targeted knockouts of genes in the nonrecombining region of the Y chromosome, including SOFF. Using elite garden asparagus lines, Agrobacterium transformations were done using reporters RUBY and GFP. A single callus indicative of RUBY expression was recovered and has begun developing shoots.