Cyclospora cayetanensis is a foodborne pathogen that causes gastrointestinal illness in humans. In 2021, 1020 cyclosporiasis cases reported in the U.S. were associated with the consumption of contaminated fresh produce. The objective of this study is to evaluate the efficiency of a proposed protocol for the detection of C. cayetanensis in fresh-cut vegetables and wash water. The new protocol differs from the conventional Bacteriological Analytical Manual (BAM) method by using an elution buffer, mini sawyer (Sawyer Products, Safety Harbor, FL) filters and nested Polymerase Chain Reaction (PCR). Oocysts were successfully collected from the Sawyer® MINI filters. Oocysts were recovered from 100g of cabbage using the elution buffer. The proposed protocol worked in detecting Cyclospora oocysts in environmental settings..