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Abstract
Conventional Salmonella surveillance requires a week for isolation, confirmation, and subsequent serotyping. To reduce this time, we sought to optimize pre-enrichment step for Salmonella recovery by addition of selective chemicals from enrichment broths to buffered peptone water (BPW). In the first study, duplicate carcass drips samples were collected immediately after feather removal in each of nine commercial processing plants (n=10). In second study, drip samples were collected at post-pick, post-inside-outside bird washer (IOBW), and post-chill; duplicate wing rinses were performed pre- and post-antimicrobial wing rinsates at three different processing plants (n=180). All the samples were cultured using traditional methods. The recovery of Salmonella using selective pre-enrichment culture conditions were similar to selective enrichment conditions (Fisher’s exact test, P1 =1; P2 = 0.72). These results suggest that a selective pre-enrichment step reduces a day in Salmonella isolation and serovar identities determined using CRISPR-SeroSeq also matched between selective pre-enrichment and enrichment methods.