Plant traits impact the dynamics of plant-arthropod interactions. Defensive plant traits have negative/positive impacts on interacting arthropods (herbivores and natural enemies). Pest management strategies focus on manipulating the expression of plant defense traits to repel herbivores and/or attract suitable biological control agents, which are considered alternative (i.e. non-insecticide) tactics to reduce pests in vegetable systems. Success in exploiting biological control agents depends on compatibility with host plants expressing defense traits. Whitefly, Bemisa tabaci (Gennadius) (Hemiptera: Aleyrodidae) control is challenging in tomato (Solanum lycopersicum L.) and is currently reliant on synthetic insecticides. To overcome this challenge, arthropod-resistant tomato plants bred with enhanced defense traits (high trichome density and acylsugar content) from wildtypes, have the potential to repel whiteflies and reduce the need for insecticides. Here I use experimental tomato lines bred to express elevated levels of acylsugar produced from glandular trichomes and predaceous mites and study their efficacy in reducing whitefly abundance in both open field and enclosed conditions. Our results suggest acylsugar-producing tomato lines provide resistance to whiteflies, but mite releases were not effective.