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Abstract
Salmonella is a food safety issue in beef products. This work investigated Salmonella prevalence and serovar populations in four cattle feedlots using deep serotyping by CRISPR-SeroSeq. The first study investigated Salmonella diversity and serovar persistence in feedlot soil layers, cattle pens, and the feedlot environment. In the second study, we assessed Salmonella transmission, between fecal and dried manure across two feedlots, for two placements. We identified 37 serovars and showed that 80% of samples contained two or more serovars. On average, samples contained 2.9 serovars (range 1 – 10). In study one, shipping/receiving pens had the highest prevalence (87.5%;14/16), and most complex samples (avg 3.9 serovars/sample). In the second study, we observed that manure samples showed higher serovar complexity than the fecal samples. Our data highlights, the enormous Salmonella complexity in feedlot environments.