A study was conducted at the University of Padova, Italy, from 2020 to 2022 to determine the impacts of mineral and organic fertilizers in a mid-term vegetable succession. The 5 fertilization treatments, i. T0 (control), ii. TMIN (100% mineral N), iii. T50 (50% mineral N 50% compost N), iv. T100 (100% compost N), and v. T200 (200% compost N), were replicated 4 times in a randomized block design. Results show the highest values for yield, quality, and nitrogen use efficiency (NUE) for TMIN. As the experiment progressed, a positive impact of compost fertilization was observed for T50 and T200, both performing better than T100. Hence, integrated application of organic and mineral fertilization or the application of double the dose of organic fertilizer is recommended while transitioning from mineral to organic fertilization to minimize the steep reduction in yield, quality, and nitrogen use efficiencies.