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Abstract
There has been no consistent plasma biomarker confirms pecan consumption. Traditionally, γ-tocopherol, a vitamin E isoform in pecans, has been previously used as a marker, peaking 5-8 hours post-consumption. However, with typical 18-24-hour testing gaps, γ-tocopherol is unsuitable as a biomarker. This single-blind, randomized crossover trial aimed to confirm whether a pecan-enriched meal provides enough substrate to possibly increase plasma urolithins (Uro), gut-metabolized products of ellagitannins. Fifteen healthy participants consumed a pecan-enriched meal and a control meal with concentrations of 16,5331 mg/mL ellagic acid (EA) 1,815.2 ng/mL γ-tocopherol and 0 mg/mL EA, 593.4 ng/mL γ-tocopherol ng/mL respectively. We measured these meals micronutrients with liquid chromatography‒tandem mass spectroscopy (LC‒MS/MS). This study purposefully matched both meals macronutrients’, but not micronutrients, to be able access if the shake recipes are adequate to examine a possible plasma Uro increase. According to our results both shakes were designed perfectly for the long run and future direction of this study.