The application of pesticides with unmanned aerial application systems (UAAS) have increased rapidly in recent years due to their potential benefits such as spot-spray herbicide applications, late-season fungicide applications in tall crops, and applications in areas inaccessible to ground sprayers. Proper selection of operational parameters including application rate, height, and speed has a significant impact on spray characteristics for both ground and aerial pesticide applications. Being a relatively new pesticide application technology, there is limited information available on the influence of different operational parameters on the spray characteristics and application efficiency of UAAS. The present work serves to evaluate the effect of varying operational parameters including rate, height, speed, and nozzle type/droplet size on spray deposition, in-swath uniformity, and drift potential for UAAS applications.