This research series assessed the optimum resistant starch (RS) nutrition in broiler chickens with emphasis on growth performance and gut health metrics as influenced by different RS sources, dietary concentrations, and feeding durations. It provided an understanding of the relationship between the metabolic products or metabolites from starch digestion and fermentation, growth, disease resistance, and tolerance responses. The first study evaluated how sources and levels of RS may influence the growth performance and nutrient utilization in broiler chickens using gut health metrics, digestion metabolites and gene expression as indices. The factors differentially modulated the nutrient digestion and energy utilization based on their botanical origins and dietary concentrations with consequences on ileal oligosaccharides and caecal SCFA profiles. Study two explored the effects of RS feeding lengths (7, 14, and 21 days) and dietary RS levels on growth performance and nutrient digestibility. RS feeding length did not significantly impact overall growth or nutrient digestibility but improved feed conversion ratio (FCR), apparent ileal digestible energy (AIDE), and metabolizable energy (ME) were observed with 35 g/kg high-amylose corn starch (HCS) outperforming 25 g/kg raw potato starch (RPS). The third study investigated the influence of optimal nutrition of resistant starch (RS) on intestinal integrity, immune response, growth performance, and broiler resistance to Eimeria challenge. The inclusion of 25 g/kg RPS and 50 g/kg HCS improved broiler growth performance during early phases, with 50 g/kg HCS yielding better results in the prepatent infection phase. Eimeria infection adversely affected growth performance during acute to compensatory phases. The apparent ileal digestibility (AID) of essential amino acids (AA) was also depressed by infection except for Trp, whereas RS diets improved the AID of Trp and Cys. These experiments provided significant insights on the use of RS to promote growth performance, disease resistance, and gut health, offering applicable strategies for the poultry industry.