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Abstract
Triple Negative Breast Cancer (TNBC) is the most aggressive type of breast cancer which disproportionately affects younger women and women of minorities. It derives its name from testing negative for the three most common breast cancer biomarkers. TNBC is currently diagnosed only at an advanced stage, necessitating the need for a diagnostic tool or method to identify this cancer at an early stage before the disease metastasizes to other parts of the body. In this research, an electrochemical biosensor was developed, optimized, and tested to detect microRNA-10b, a promising biomarker for TNBC. The biosensor is able to detect 1 nM of microRNA-10b in laboratory serum samples, which is sufficient enough for early diagnosis of TNBC. This biosensor proved to be specific to the target biomarker compared to other non-target microRNAs of similar size. The efficacy of the biosensor for TNBC early diagnosis was tested using human serum samples.
INDEX WORDS: Impedance, MicroRNA, Biomarker, Breast Cancer, Detection Method, Rapid Diagnostic, Graphene
INDEX WORDS: Impedance, MicroRNA, Biomarker, Breast Cancer, Detection Method, Rapid Diagnostic, Graphene