Salmonellosis, a zoonotic disease caused by Salmonella, is primarily attributed to the consumption of poultry eggs and meat. Salmonella enterica serovars enteritis (SE) and heidelberg (SH) are among the most frequent serotypes recovered from humans. Within few hours after chickens are orally infected, Salmonella can invade the intestinal tract and reach internal organs. Reducing SE or SH colonization in poultry may lead to a decrease of its transfer to humans, resulting in fewer outbreaks. Vaccines have proven to be a useful tool to control Salmonella. Their early protective efficacy is primarily conferred by the induction of antigen-specific antibodies. Killed Salmonella vaccines eliminate the probability of the live strain to regain its virulence, while oral administration of antigens efficiently stimulate mucosal and systemic immune responses. However, no oral killed vaccines are currently commercially available for broiler. This project characterizes the protective effects of an oral Chitosan Nanoparticle-Salmonella vaccine using broiler birds.