PURPOSE: The purpose of this study was to measure quadriceps and hamstring strength, endurance, and mitochondrial capacity in female collegiate athletes that have undergone ACL reconstructive surgery on a single leg. METHODS: 10 subjects were included in this study. Quadricep and hamstring strength were measured using data collected from a Biodex Multi-Joint System isokinetic dynamometer. Muscle endurance was measured using data from accelerometers. Muscle mitochondrial capacity was measured using near-infrared spectroscopy (NIRS) Portamon devices. All testing was completed in single session. RESULTS: There was not a significant difference of strength for injury (F = 3.65, p = 0.088). Muscular endurance did not demonstrate a significant difference for injury (F = 1.137, p = 0.314). Mitochondrial capacity was not different in quadriceps or hamstring for injured and non-injured legs (F = 4.169, p = 0.055). CONCLUSIONS: This study did not demonstrate impairments in mitochondria or endurance. However, these measures remain important in rehabilitation from knee injury.