Files
Abstract
The glutathione (GSH) antioxidant system governs cellular protection against oxidative stress, which is characterized by depletion of GSH and subsequent accumulation of the dimer glutathione disulfide (GSSG). Previous research indicates a negative effect of a high-fat diet on GSH redox status (GSH/GSSG) and a possible protective effect of certain flavonoids such as anthocyanins. Although tissue levels of GSH, GSSG, and GSH/GSSG are also heritable, it is unclear whether genetic background and diet interact to regulate the GSH system. The current work tested the hypothesis that a high-fat diet regulates GSH homeostasis in a manner dependent on genetic background. Additionally, it tested the hypothesis that anthocyanin-induced alterations in GSH homeostasis are genetically determined. Both hypotheses were confirmed, indicating that a high-fat diet and anthocyanin supplementation interact with genetic background to regulate the GSH system and subsequent chronic disease risk.