The U.S. poultry industry uses an average of 26 L (7 gal) of potable water per broiler during processing. Each step in the process generates byproducts that combine with this water to form the facilitys poultry processing wastewater (PPW) stream. While extensive research has been conducted on concentration (mg/L) of constituents in PPW since the 1950s, little data exists on the impact that the various processing byproducts have on PPW as measured by wastewater stream loading (g/kglwt). Also, little is known about the variation in impact that individual broilers have on PPW. Experiments were conducted to establish the variation in PPW loading, as well as determining which byproducts have the greatest PPW impact and at which points in the slaughter process the greatest impact occurs. Samples of scalder PPW, and feather and viscera rinse PPW were analyzed for common wastewater parameters (e.g., COD, TS, TSS, TVS, and TKN). Results demonstrated that bleeding time, external debris, and transport time of slaughter byproducts in offal flumes significantly increased the organics, solids and nutrient loading in PPW.