The native herbaceous plant, Hibiscus moscheutos L., was used in this project. Polyploidy induction was attempted via soaking seeds and seedlings in colchicine and oryzalin. Both colchicine and oryzalin were effective in inducing tetraploids. Triploids were obtained from the traditional method of crossing tetraploids with diploids. Triploid and tetraploid plants showed varying degrees of difference from diploid plants when comparing morphological traits. Shoots of H. moscheutos were also treated in colchicine and oryzalin in tissue culture for polyploidy induction. Only one tetraploid was successfully induced in tissue culture in varying concentrations of mitotic inhibitors during different exposure durations. Glutamine was added in tissue culture media to compare with some common PGRs in stimulating shoot elongation. After seven weeks of culture, glutamine promoted shoot elongation inconsistently in different studies. More experimentation should be attempted with other amino acids as a nitrogen source for promoting plant growth in vitro.