In the post-Chernobyl world, the evaluation of past, present, and future impacts of chronic radiation exposure on the environment is of utmost importance. The Fukushima Exclusion Zone (FEZ) created following the Fukushima Daiichi Nuclear Disaster provides a unique opportunity to study potential impacts of radiation on terrestrial wildlife in an area from which humans have been evacuated. Through the use of remote-activated cameras, we were able to quantify the distribution, relative abundance, and activity patterns of wildlife across a gradient of radioactive contamination and human habitation. Additionally, via deployment of GPS collars, we quantified the home ranges and resource selection of masked palm civets (Paguma larvata) and raccoons (Procyon lotor). We found that species occupancy and abundance was most strongly determined not by radiation, but by the extent of human presence and environmental characteristics such as elevation and habitat type. The impact of human presence on the distribution, abundance, and activity of wildlife, was most pronounced for generalist species routinely in conflict with humans, particularly wild boar. We also found that radiation had no discernable influence on resource selection patterns of masked palm civets or raccoons, but rather the space use of these species was primarily driven by the availability of preferred habitats, including extensive use of abandoned structures and crops within the FEZ.