Circulating microparticles influence vascular homeostasis by participating in angiogenesis, fibrinolysis, coagulation, and endothelial function. Microparticles can be used to characterize and understand the effect of exercise on vascular health. We investigated the effect of an acute bout of continuous and interval exercise on CD62E+ endothelial microparticles (EMPs) and CD34+ microparticles (MPs) in men and women. Blood samples were collected at baseline, halfway through exercise, immediately after exercise, and at 30, 60, 90, and 120 minutes after exercise. Interval exercise consisted of ten, 1-minute intervals at 100% and 90% VO2max on the treadmill. The continuous bout was energy matched to the interval bout, and lasted ~22 minutes at 65% VO2max. Overall, exercise produced a decrease from baseline in CD62E+ EMPs, however there was no effect of exercise on CD34+ MPs. This reduction in CD62E+ EMPs with exercise could be contributing to the overall positive effect of exercise on cardiovascular health.