Human-wildlife interactions (HWI) are a significant concern for biodiversity conservation in Costa Rica. Despite the rich biodiversity in this ecologically diverse country, particularly within biological corridors, HWIs have not been comprehensively studied and there are few well-documented examples that illustrate the continuum of HWIs with both positive and negative outcomes. Populations of the Endangered Baird’s tapir (Tapirus bairdii), the largest native-terrestrial mammal, are primarily concentrated in protected areas across their distribution range. However, recent reports indicate an increase in tapirs using landscapes outside protected areas, particularly in regions with strong community-based conservation initiatives like the Tenorio-Miravalles Biological Corridor (TMBC) in Northwestern Costa Rica. Here, human-tapir interactions are common, and human-tapir conflicts have been characterized by poaching events, by the increase on recent reports of tapirs that are injured by vehicles, by inappropriate handling, or that are also prosecuted by people in retaliation for raiding crops, because farmers suffer economically from crop damage. Through this study, I comprehensively investigated the human-tapir interactions occurring at TMBC, with an integration of an evaluation of the key governance and landscape connectivity factors influencing tapir conservation, in addition to an assessment of the Payment for Ecosystem Services (PSA) scheme at TMBC. I identified hotspots of human-tapir conflicts by employing hierarchical single-species spatial occupancy and activity pattern models to assess tapir habitat use, activity diel and seasonal patterns, and to predict the locations of potential human-tapir interactions. Additionally, I collected data on past poaching events and farmer-tapir conflicts from local authorities and conducted road surveys to identify vehicle-tapir collision hotspots. I also investigated tapir space use, habitat selection, and movement patterns of tapirs at TMBC by employing integrated Step Selection Functions (iSSFs) based on GPS radiocollar movement data. I evaluated the landscape connectivity by using Omniscape, and for the governance aspect, I described the local governance through the Management Territorial Plan, and the management at TMBC and associated protected areas, and characterized the PSA scheme program. Tapir occupancy was highest in forest fragments, in proximity to Tenorio Volcano National Park (TNP), and in flat areas. Tapir activity patterns revealed that tapirs had mostly crepuscular activity between 19:00 h and 22:00 h, reaching a peak of maximum activity within these hours. Although the seasonal variation in tapir activity was not significant, I found more detections in April, at the beginning of the rainy season. These results suggest that environmental factors and anthropogenic activities related to protected area management, such as forest fragments and proximity to TNP, significantly influence tapir habitat use within the TMBC. The predictions identified potential conflict zones within the corridor. Historical data on poaching events and farmer-tapir conflicts provided by local authorities and road surveys indicated specific hotspots of human-tapir interactions along major roads within the TMBC, characterized by frequent tapir sightings or non-fatal vehicle-tapir collisions. Key findings indicated several areas with prevalent human-tapir conflicts. Movement results revealed significant variations in individual home range sizes among tapirs. Females exhibited significant variability in habitat selection, while males preferred habitats near water bodies and roads, as well as pastures at night. Movement patterns revealed that our individuals monitored do not have a persistent movement, while showing variation of movement rates. The landscape connectivity analysis indicated that the normalized cumulative current indicated several bottlenecks of flow located throughout the central and northern TMBC. Results from the road survey revealed a lack of road mitigation measures and wildlife-friendly infrastructure at TMBC, where tapirs and other species are in constant risk of collisions with vehicles. This research highlights the importance of biological corridors for the connectivity of tapir populations between protected areas. These findings offer valuable insights for landscape management and planning, emphasizing the need to protect and restore riparian habitat fragments to promote species conservation and enhance corridor connectivity. Conservation efforts should consider the habitat use and movement preferences of tapirs to ensure species long-term survival including protection and restoration of riparian forest fragments and of buffer areas around protected areas, increase the implementation of road mitigation measures (e.g., wildlife road-crossing signs, speed bumps, underpasses), and of driver awareness, and augment “tapir-friendly” agriculture practices (e.g., electric fencing to repel tapirs from raiding crops, low-pesticide use) to promote human-tapir coexistence.