New transgenic technology (Bollgard II/Roundup Ready Flex) will continue to revolutionize cotton production. A two year study (2004-2005) was conducted at two locations in southwest Georgia to test the responses of Bollgard II/Roundup Ready Flex (BGII/RRF) and Bollgard/Roundup Ready (BG/RR) to water stress, flower removal (FR), and late glyphosate applications. Late glyphosate applications delayed maturity in BG/RR cotton, which compensated for fruit loss by producing heavier remaining bolls, while BGII/RRF cotton produced a higher number of bolls per 10 plants. The BGII/RRF cotton had increased boll number and weight at the first sympodial position at lower main stem nodes, while BG/RR produced more and heavier bolls on the upper main stem nodes. Flower removal did not negatively affect BGII/RRF or BG/RR, further supporting the hypothesis that compensation for early fruit loss may occur. Few differences in fiber quality were observed.