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Abstract
A chemoenzymatic approach has been developed for the preparation of diverse libraries of heparan sulfate (HS) oligosaccharides. It employs chemically synthesized oligosaccharides having a chemical entity at a GlcN residue, which in unanticipated manners influences the site of modification by NST, C5Epi/2OST and 6OST1/6OST3, thus resulting in oligosaccharides differing in N/Osulfation and epimerization pattern. The enzymatic transformations defined fine substrate requirements of NST, C5Epi, 2OST, and 6OST.