This study aims to biologically characterize low pathogenic avian influenza (LPAI) wild bird isolates in selected domestic avian species. Groups of specific pathogen-free chickens and commercial turkeys were intrachoanally infected with 16 LPAI viruses. The pathogenesis, virus shedding and serologic response of these species were determined through clinical signs and histopathological lesions, rRT-PCR and ELISA and HI tests, respectively. All viruses did not cause significant clinical disease except for MN 99-263 in turkeys. Analyses of oropharyngeal and cloacal swabs demonstrated greater virus shedding from both anatomical sites in chickens compared to turkeys. Respiratory and gastro-intestinal tract lesions were predominant in both species, confirming the low pathogenicity of these viruses. More LPAI isolates caused seroconversion in turkeys than in chickens, emphasizing the greater susceptibility of turkeys to AIV infection. This study indicates that most LPAI wild bird isolates can be shed by infected birds without causing overt clinical disease or mortality.