Ready to eat meat products have been the source for the isolation of some LAB which are known to produce metabolic byproducts with inhibitory activity against spoilage and pathogenic bacteria. Leuconostoc carnosum 4010 was selected as the antimicrobial agent (cells and metabolic products) to be incorporated into an edible film. MIC was 1 ml of LAB cell-free 2supernatant against ca. 100 CFU/cm of Listeria. Inhibitory activity was a synergistic effect of the bacteriocin and lactic acid. Viable LAB cells had an effect after 21 days at 4C. The antimicrobial film C, 0.51 0.02 mm thick, demonstrated the highest inhibitory action against L. 2monocytogenes. After 14 days of treatment, almost 2 log10 CFU/25 cm reductions were 32achieved from initial Listeria inoculation of 10 CFU/25 cm. Antimicrobial film is a promising form of active food packaging as a response to the increasing demand for natural and minimally processed foods.