Lipids are prevalent in nature and serve various functions at the cellular level. For instance, they make up more than fifty percent of the cell membrane, which serves as a barrier between the inner contents of the cell and the external environment. This membrane must allow the entry of nutrients into the cell and excretion of waste out of the cell with little perturbation to the overall cellular structure. The bending rigidity of lipids is one of the most investigated property of lipids because it governs the stiffness of a bilayer membrane. In this work, we experimentally investigated the bending rigidity of bilayer membranes consisting of 1,2-diphytanoyl-sn-glycero-3-phosphocholine (DPhPC) lipids, and compared it to that of Molecular Dynamic (MD) Coarse-Grained simulations. We used the electroformation technique to generate Giant Unilamellar Vesicles (GUVs), and the minimally intrusive fluctuation analysis technique to obtain the bending rigidity.