This thesis offers an analysis of how the Gospel of John uses and interprets Second Isaiah. In particular, it addresses how and why the Fourth Gospel uses a trial motif, as well as the function and significance of the I am statements. Due to the nature of the investigation, it is necessary to work from a relatively broad understanding of dependence and background. I frequently return to Richard Bauckhams approach to Second Temple Judaisms understanding of God by means of a unique divine identity as a methodological basis. David Balls literary critical approach, as well as Andrew Lincolns understanding of the trial motif, is also given a majority of attention. Their ideas are then brought into dialogue with other scholars. The conclusion of this study highlights the evangelists creative reworking of the inherited Jesus traditions, contemporary Jewish thought, and the Jewish Scriptures of which Second Isaiah played a major role.