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Abstract
We examined the roles of histone-DNA interactions and histone H1 in regulatingchromatin structure in Arabidopsis thaliana and Neurospora crassa. Using K-means clustering, we categorized Arabidopsis genes based on their nucleosome organization. Results showed that for a subset of actively transcribed genes lacked stereotypical organization with no discernible consequences on transcriptional level. This difference in organization seems to be predicted by the surrounding DNA sequence and may be related to transcriptional consistency and inducibility. We also examined the effects on chromatin structure in Neurospora following the loss of histone H1. Unexpectedly we saw little difference in nucleosome organization in the H1 deletion mutant; however these result should not discount the potential role of histone H1 in regulation chromatin structure in other organisms.