Drosophila suzukii and Anopheles gambiae are of great concern to humans as they are important agricultural pests and vectors of human diseases, respectively. D. suzukii is a devastating pest of soft and thin-skinned fruits. It causes substantial economic losses to fruit production industries, forcing farmers to apply broad-spectrum insecticides frequently which could lead to the development of insecticide resistance, thus jeopardizing their efficacy. The LC50 values of zeta-cypermethrin, spinosad, and malathion insecticides were determined against a Pierce and a Clarke county D. suzukii population. The LC50 values were three fold higher in the Pierce population for all treatments. Furthermore, RNA sequencing approach was used to analyze the response of Pierce and Clarke D. suzukii population at the transcriptome level upon insecticide treatments. A higher number of genes involved in detoxification and reduced cuticular penetration, were differentially expressed in the Pierce population. Finally, fewer nonsynonymous single nucleotide variants with deleterious effect on protein function were predicted among detoxification, and cuticular protein encoding genes in Pierce flies. Thus a combination of increased gene expression and fewer deleterious SNVs suggest molecular mechanisms underlying the higher LC50 values for Pierce population. Anopheles gambiae is an important vector of human malaria and o'nyong-nyong fever. The complexity of handling A. gambiae and infectious pathogens has led to the use of A. gambiae cell lines, including Ag55 cells, as a potential model to study vector-pathogen interactions and immune responses. The utility of cell lines can be maximized if their detailed gene expression profile, and proteome are available. Omics approaches were applied to provide a detailed gene expression profile and proteome of Ag55 cells. The transcriptome of Ag55 cells was compared to that of blood fed female adults to establish real patterns of gene expression which indicate specific functions of Ag55 cells. Gene Ontology enrichment analysis of enriched transcript suggested that Ag55 cells have phagocytic properties, a hypothesis which was confirmed by the ability of Ag55 cells to phagocytize E. coli bioparticles. Transcriptomic data further backed by proteomic data suggest that Ag55 cells express hemocyte like properties.