This study investigated the relationships among organization characteristics,organizational culture, journalistic professional culture, and news organizations adoption of user-generated content (UGC) in the United States. This study used a cross-sectional survey design to explore the dominant culture in daily newspapers and broadcast network-affiliated television stations, journalists core values of professionalism, and to what extent newsorganizations adopted UGC. The study found organizational culture played an important role in the process of UGC adoption by news organizations. The more a news organization emphasized the values of adhocracy culture that is characterized by a creative and dynamic workplace, the higher level itsUGC adoption was. However, the study did not find a significant relationship between journalistic professional culture and news organizations UGC adoption level. Moreover, the study found some organization characteristics like organization size and organization longevity were related to news organizations UGC adoption level. In one word, the findings of the studysuggested that organizational culture and some organization characteristics are more important than professional culture of journalism in the process of UGC adoption by legacy mediaorganizations.