Structured lipids (SLs) can be used in infant formulas as human milkfat analogues to mimic the fatty acid composition of human breast milk. The purpose of this study was to produce a SL with a high content of sn-2 palmitic acid from anhydrous milkfat enriched with docosahexaenoic (DHA) and arachidonic (ARA) acids using lipases as biocatalysts. The oxidative stability of the SL was studied in complex oil in water (O/W) infant formula emulsions with added antioxidants. The total percentages of ARA and DHA contained in the SL were 0.63 and 0.50 mol% with 0.55 and 0.46 mol% at the sn-2 position, respectively. It also contained 44.97 mol% of sn-2 palmitic acid. The oxidative stability of the SL in O/W emulsions was improved using a glucose-cysteine Maillard reaction product and other antioxidants including tocopherol and ascorbyl palmitate. This SL might be totally or partially used in fat blends for infant formula.