Levels of moisture stress impact herbicidal uptake and translocation by plants. The objective of this research was to observe the effects moisture stress has on greenhouse grown tropical spiderwort plants and also to observe the effects of herbicidal control, uptake and translocation. Leaf characterization was observed under a scanning electron microscope to determine differences caused by moisture stress. Treatments were subjected to three different moisture stress levels (25, 50 and 100% of field capacity), and sprayed post emergence with selected herbicides. Translocation of diclosulam, imazapic and S-metolachlor into the roots and shoots was observed. Foliar uptake of diclosulam and imazapic were not affected by changes in moisture stress. Studies were conducted to observe the effects of varying rates of different herbicides on aerial and underground seed. S-metolachlor had the greatest control of aerial and underground seed.