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Abstract
River cane [Arunindaria gigantea (Walter) Muhl.] is an endangered species of endemic North American bamboo. Because of its ability to effectively control erosion along waterways and function as a riparian buffer, restoration of river cane is of great importance. However, there have been few investigations into propagation methods for river cane. Here a method of micropropagation on nodal segments was initiated and showed that 7 mg/L of benzyladenine produced superior shoot proliferation in vitro. To further inform restoration, the genetic population structure of three river cane population was investigated. Six simple sequence repeat loci and sequencing of three chloroplast regions showed that each population contains a dominant genotype accounting for 66%-93% of individuals, and genotypes are shared across populations, indicating low differentiation among populations