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Abstract
The influence of lysine and phosphorus deficiency on chicken immune function was conducted with broiler chickens fed lysine-deficient diets at 65% of the control diet and phosphorus-deficient diets at 67% of the control diet. The evaluation of humoral immune function was evaluated by measuring the antibody production to Newcastle disease vaccination using the hemagglutination inhibition (HI) test and enzyme-linked immunosorbent assay (ELISA). The cellular immune function was evaluated by cutaneous basophile hypersensitivity and lymphocyte proliferation test. Result of lysine deficiency indicates reduced humoral immune function measured by ELISA but not by HI and the cell-mediated immune function also was reduced by lysine deficiency. Result of phosphorus deficiency indicates that phosphorus deficiency had no great influence on chicken immune function under the parameters of this study.