The reduction in plant height is imparted by the gene d2, which is located on linkagegroup 4 (LG4) in pearl millet. In order to fine-map d2, a large F2 mapping population was generated from the cross ICMP 451 (D2D2) x Tift 23DB (d2d2) and genotyped with markers B224C4P2 and PSM305 to identify plants that carry a recombination event in the d2 region. Markers that mapped to the d2 region in a previously constructed low-density map were mappedprecisely using 25 recombinant plants. Additional markers were developed from the sorghumgenomic sequence and mapped to the d2 region. Based on the phenotypic and genotypic data ofthe ICMP 451 X Tift 23DB cross, and the genotypic data of a second cross between PT732Band P1449-2, the physical location of d2 can be narrowed down to the interval spannedproximally by the MDH/Gene 44/ PLATZ marker cluster and distally by PSM 344 on LG4.
