Influenza A virus (IAV) vaccination of animals and humans is a powerful tool for prevention and control of disease. Currently licensed vaccines are egg-based and delivered by injection. Baculovirus grown in insect cells cultures can produce high yields of virus-like particles (VLP). VLP vaccines have been shown to be highly immunogenic after parenteral application in mice, ferrets, and humans. The aim of this study was to assess influenza VLPs, as an aerosolized vaccine, as a vaccination strategy in chickens. One-day-old SPF chickens were vaccinated twice with 100 ug, 20 ug, or 5 ug of VLPs. As control, chickens were also vaccinated via intranasal instillation and intramuscular injection. The VLPs induced seroconversion after intramuscular application at any dosage. In contrast, aerolized VLPs induced a specific antibody response after aerosolization but only when 100 ug were given. These data show that influenza VLPs might be used for mass aerosol vaccination in chickens.